PONTIFICAL ACADEMY FOR LIFE
DECLARATION ON THE PRODUCTION AND THE SCIENTIFIC AND
THERAPEUTIC USE OF HUMAN EMBRYONIC STEM CELLS
August 25, 2000.
This document seeks to contribute to the debate on the production and use of
embryonic stem cells which is now taking place in scientific and ethical
literature and in public opinion. Given the growing relevance of the debate on
the limits and liceity of the production and use of such cells, there is a
pressing need to reflect on the ethical implications which are present.
The first section will very briefly set out the most recent scientific data
on stem cells and the biotechnological data on their production and use. The
second section will draw attention to the more relevant ethical problems raised
by these new discoveries and their applications.
Although some aspects need to be studied more thoroughly, a commonly accepted
definition of Astem cell" describes it as a cell with two characteristics: 1)
the property of an unlimited self-maintenance - that is, the ability to
reproduce itself over a long period of time without becoming differentiated; and
2) the capability to produce non-permanent progenitor cells, with limited
capacity for proliferation, from which derive a variety of lineages of highly
differentiated cells (neural cells, muscle cells, blood cells, etc.). For about
thirty years stem cells have provided a vast field of research in adult
tissue,[i] in embryonic tissue and in in vitro cultures of embryonic stem cells
of experimental animals.[ii] But public attention has recently increased with a
new milestone that has been reached: the production of human embryonic stem
Human embryonic stem cells
Today, the preparation of human embryonic stem cells (human ES cells) implies
the following[iii]: 1) the production of human embryos and/or the use of the
surplus embryos resulting from in vitro fertilization or of frozen embryos; 2)
the development of these embryos to the stage of initial blastocysts; 3) the
isolation of the embryoblast or inner cell mass (ICM) - which implies the
destruction of the embryo; 4) culturing these cells on a feeder layer of
irradiated mouse embryonic fibroblasts in a suitable medium, where they can
multiply and coalesce to form colonies; 5) repeated subculturing of these
colonies, which lead to the formation of cell lines capable of multiplying
indefinitely while preserving the characteristics of ES cells for months and
These ES cells, however, are only the point of departure for the preparation
of differentiated cell lines, that is, of cells with the characteristics proper
of the various tissues (muscle, neural, epithelial, haematic, germinal, etc.).
Methods for obtaining them are still being studied;[iv] but the injection of
human ES cells into experimental animals (mice) or their culture in vitro in
controlled environments to their confluence have shown that they are able to
produce differentiated cells which, in a normal development, would derive from
the three different embryonic tissue layers: endoderm (intestinal epithelium),
mesoderm (cartilage, bone, smooth and striated muscle) and ectoderm (neural
epithelium, squamous epithelium).[v]
The results of these experiments had a great impact on the world of both
science and biotechnology - especially medicine and pharmacology - no less than
the world of business and the mass media. There were high hopes that the
application of this knowledge would lead to new and safer ways of treating
serious diseases, something which had been sought for years.[vi] But the impact
was greatest in the political world.[vii] In the United States in particular, in
response to the long-standing opposition of Congress to the use of federal funds
for research in which human embryos were destroyed, there came strong pressure
from the National Institutes of Health (NIH), among others, to obtain funds for
at least using stem cells produced by private groups; there came also
recommendations from the National Bioethics Advisory Committee (NBAC),
established by the Federal Government to study the problem, that public money
should be given not only for research on embryonic stem cells but also for
producing them. Indeed, persistent efforts are being made to rescind
definitively the present legal ban on the use of federal funds for research on
Similar pressures are being brought to bear also in England, Japan and
It had become clear that the therapeutic use of ES cells, as such, entailed
significant risks, since - as had been observed in experiments on mice - tumours
resulted. It would have been necessary therefore to prepare specialized lines of
differentiated cells as they were needed; and it did not appear that this could
be done in a short period of time. But, even if successful, it would have been
very difficult to be certain that the inoculation or therapeutic implant was
free of stem cells, which would entail the corresponding risks. Moreover there
would have been a need for further treatment to overcome immunological
incompatibility. For these reasons, three methods of therapeutic cloning[viii]
were proposed, suitable for preparing pluripotent human embryonic stem cells
with well defined genetic information from which desired differentiation would
1. The replacement of the nucleus of an oocyte with the nucleus of an adult
cell of a given subject, followed by embryonic development to the stage of
blastocyst and the use of the inner cell mass (ICM) in order to obtain ES cells
and, from these, the desired differentiated cells.
2. The transfer of a nucleus of a cell of a given subject into an oocite of
another animal. An eventual success in this procedure should lead - it is
presumed - to the development of a human embryo, to be used as in the preceding
3. The reprogramming of the nucleus of a cell of a given subject by fusing
the ES cytoplast with a somatic cell karyoplast, thus obtaining a "cybrid". This
is a possibility which is still under study. In any event, this method too would
seem to demand a prior preparation of ES cells from human embryos.
Current scientific research is looking to the first of these possibilities as
the preferred method, but it is obvious that - from a moral point of view, as we
shall see - all three proposed solutions are unacceptable.
Adult stem cells
From studies on adult stem cells (ASC) in the last thirty years it had been
clearly shown that many adult tissues contain stem cells, but stem cells capable
of producing only cells proper to a given tissue. That is, it was not thought
that these cells could be reprogrammed. In more recent years,[ix] however,
pluripotent stem cells were also discovered in various human tissues - in bone
marrow (HSCs), in the brain (NSCs), in the mesenchyme (MSCs) of various organs,
and in umbilical cord blood (P/CB, placental/cord blood); these are cells
capable of producing different types of cells, mostly blood cells, muscle cells
and neural cells. It was learnt how to recognize them, select them, maintain
them in development, and induce them to form different types of mature cells by
means of growth factors and other regulating proteins. Indeed noteworthy
progress has already been made in the experimental field, applying the most
advanced methods of genetic engineering and molecular biology in analyzing the
genetic programme at work in stem cells,[x] and in importing the desired genes
into stem cells or progenitor cells which, when implanted, are able to restore
specific functions to damaged tissue.[xi] It is sufficient to mention, on the
basis of the reported references, that in human beings the stem cells of bone
marrow, from which the different lines of blood cells are formed, have as their
marker the molecule CD34; and that, when purified, these cells are able to
restore entirely the normal blood count in patients who receive ablative doses
of radiation and chemotherapy, and this with a speed which is in proportion to
the quantity of cells used. Furthermore, there are already indications on how to
guide the development of neural stem cells (NSCs) through the use of various
proteins - among them neuroregulin and bone morphogenetic protein 2 (BMP2) -
which can direct NSCs to become neurons or glia (myelin-producing neural support
cells) or even smooth muscle tissue.
The note of satisfaction, albeit cautious, with which many of the cited works
conclude is an indication of the great promise that Aadult stem cells" offer for
effective treatment of many pathologies. Thus the affirmation made by D. J. Watt
and G. E. Jones: A The muscle stem cell, whether it be of the embryonic myoblast
lineage, or of the adult satellite status, may well turn out to be a cell with
far greater importance to tissues other than its tissue of origin and may well
hold the key to future therapies for diseases other than those of a myogenic
nature" (p. 93). As J. A. Nolta and D. B. Kohn emphasize: AProgress in the use
of gene transfer into haemotopoietic cells has led to initial clinical trials.
Information developed by these early efforts will be used to guide future
developments. Ultimately, gene therapy may allow a number of genetic and
acquired diseases to be treated, without the current complications from bone
marrow transplantation with allogeneic cells." (p. 460); and the confirmation
offered by D. L. Clarke and J. Frisén: "These studies suggest that stem cells in
different adult tissues may be more similar than previously thought and perhaps
in some cases have a developmental repertoire close to that of ES cells" (p.
1663) and Ademonstrates that an adult neural stem cell has a very broad
developmental capacity and may potentially be used to generate a variety of cell
types for transplantation in different diseases@ (p. 1660).
The progress and results obtained in the field of adult stem cells (ASC) show
not only their great plasticity but also their many possible uses, in all
likelihood no different from those of embryonic stem cells, since plasticity
depends in large part upon genetic information, which can be reprogrammed.
Obviously, it is not yet possible to compare the therapeutic results obtained
and obtainable using embryonic stem cells and adult stem cells. For the latter,
various pharmaceutical firms are already conducting clinical experiments[xii]
which are showing success and raising genuine hopes for the not too distant
future. With embryonic stem cells, even if various experimental approaches prove
positive,[xiii] their application in the clinical field - owing precisely to the
serious ethical and legal problems which arise - needs to be seriously
reconsidered and requires a great sense of responsibility before the dignity of
every human being.
Given the nature of this article, the key ethical problems implied by these
new technologies are presented briefly, with an indication of the responses
which emerge from a careful consideration of the human subject from the moment
of conception. It is this consideration which underlies the position affirmed
and put forth by the Magisterium of the Church.
The first ethical problem, which is fundamental, can be formulated thus: Is
it morally licit to produce and/or use living human embryos for the preparation
of ES cells?
The answer is negative, for the following reasons:
1. On the basis of a complete biological analysis, the living human embryo is
- from the moment of the union of the gametes - a human subject with a well
defined identity, which from that point begins its own coordinated, continuous
and gradual development, such that at no later stage can it be considered as a
simple mass of cells.[xiv]
2. From this it follows that as a Ahuman individual" it has the right to its
own life; and therefore every intervention which is not in favour of the embryo
is an act which violates that right. Moral theology has always taught that in
the case of Ajus certum tertii" the system of probabilism does not apply.[xv]
3. Therefore, the ablation of the inner cell mass (ICM) of the blastocyst,
which critically and irremediably damages the human embryo, curtailing its
development, is a gravely immoral act and consequently is gravely illicit.
4. No end believed to be good, such as the use of stem cells for the
preparation of other differentiated cells to be used in what look to be
promising therapeutic procedures, can justify an intervention of this kind. A
good end does not make right an action which in itself is wrong.
5. For Catholics, this position is explicitly confirmed by the Magisterium of
the Church which, in the Encyclical Evangelium Vitae, with reference to the
Instruction Donum Vitae of the Congregation for the Doctrine of the Faith,
affirms: AThe Church has always taught and continues to teach that the result of
human procreation, from the first moment of its existence, must be guaranteed
that unconditional respect which is morally due to the human being in his or her
totality and unity in body and spirit: >The human being is to be respected and
treated as a person from the moment of conception; and therefore from that same
moment his rights as a person must be recognized, among which in the first place
is the inviolable right of every innocent human being to life'"(No. 60).[xvi]
The second ethical problem can be formulated thus: Is it morally licit to
engage in so-called Atherapeutic cloning" by producing cloned human embryos and
then destroying them in order to produce ES cells?
The answer is negative, for the following reason: Every type of therapeutic
cloning, which implies producing human embryos and then destroying them in order
to obtain stem cells, is illicit; for there is present the ethical problem
examined above, which can only be answered in the negative.[xvii]
The third ethical problem can be formulated thus: Is it morally licit to use
ES cells, and the differentiated cells obtained from them, which are supplied by
other researchers or are commercially obtainable?
The answer is negative, since: prescinding from the participation - formal or
otherwise - in the morally illicit intention of the principal agent, the case in
question entails a proximate material cooperation in the production and
manipulation of human embryos on the part of those producing or supplying them.
In conclusion, it is not hard to see the seriousness and gravity of the
ethical problem posed by the desire to extend to the field of human research the
production and/or use of human embryos, even from an humanitarian perspective.
The possibility, now confirmed, of using adult stem cells to attain the same
goals as would be sought with embryonic stem cells - even if many further steps
in both areas are necessary before clear and conclusive results are obtained -
indicates that adult stem cells represent a more reasonable and human method for
making correct and sound progress in this new field of research and in the
therapeutic applications which it promises. These applications are undoubtedly a
source of great hope for a significant number of suffering people.
Prof. Juan de Dios Vial Correa
The Vice President
S.E. Mons. Elio Sgreccia
Vatican City, August 25, 2000.
[i].Cf. M. LOEFFLER, C. S POTTEN, Stem Cells and Cellular Pedigrees - a
Conceptual Introduction, in C. S. POTTEN (ed.), Stem Cells, Academic Press,
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2000, 287, 1439-1441.
[ii].Cf. T: NAKANO, H. KODAMA, T. HONJO, Generation of Lymphohematopoietic
Cells from Embryonic Stem Cells in Culture, Science 1994, 265, 1098-1101; G.
KELLER, In Vitro Differentiation of Embryonic Stem Cells, Current Opinion in
Cell Biology 1995, 7, 862-869; S. ROBERTSON, M. KENNEDY, G. KELLER,
Hematopoietic Commitment During Embryogenesis, Annals of the New York Academy of
Sciences 1999, 872, 9-16.
[iii].Cf. J. A .THOMSON, J. ITSKOVITZ-ELDOR, S. S. SHAPIRO et al., Embryonic
Stem Cell Lines Derived from Human Blastocysts, Science 1998, 282, 1145-1147; G.
VOGEL, Harnessing the Power of Stem Cells, Science 1999, 283, 1432-1434.
[iv].Cf. F. M. WATT, B. L. M. HOGAN, Out of Eden: Stem Cells and Their
Niches, Science 2000, 287, 1427-1430.
[v].Cf. J. A. THOMSON, J. ITSKOVITZ-ELDOR, S. S. SHAPIRO et al., op. cit.
[vi].Cf. U.S. CONGRESS, OFFICE OF TECHNOLOGY ASSESSMENT, Neural Grafting:
Repairing the Brain and Spinal Cord, OTA-BA-462, Washington, DC, U.S. Government
Printing Office, 1990; A. McLAREN, Stem Cells: Golden Opportunities with Ethical
Baggage, Science 2000, 288, 1778.
[vii].Cf. E. MARSHALL, A Versatile Cell Line Raises Scientific Hopes, Legal
Questions, Science 1998, 282, 1014-1015; J. GEARHART, New Potential for Human
Embryonic Stem Cells, ibid., 1061-1062; E. MARSHALL, Britain Urged to Expand
Embryo Studies, ibid., 2167-2168; 73 SCIENTISTS, Science Over Politics, Science
1999, 283, 1849-1850; E. MARSHALL, Ethicists Back Stem Cell Research, White
House Treads Cautiously, Science 1999, 285, 502; H. T. SHAPIRO, Ethical Dilemmas
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Embryonic Stem Cell Research, Science 1999, 286, 2050; G. KELLER, H. R.
SNODGRASS, Human Embryonic Stem Cells: the Future Is Now, Nature Medicine 1999,
5, 151-152; G.J. ANNAS, A. CAPLAN, S. ELIAS, Stem Cell Politics, Ethics and
Medical Progress, ibid., 1339-1341; G. VOGEL, Company Gets Rights to Cloned
Human Embryos, Science 2000, 287, 559; D. NORMILE, Report Would Open Up Research
in Japan, ibid., 949; M. S. FRANKEL, In Search of Stem Cell Policy, ibid., 1397;
D. PERRY, Patients Voices: the Powerful Sound in the Stem Cell Debate, ibid.,
1423; N. LENOIR, Europe Confronts the Embryonic Stem Cell Research Challenge,
ibid., 1425-1427; F. E. YOUNG, A Time for Restraint, ibid., 1424; EDITORIAL,
Stem Cells, Nature Medicine 2000, 6, 231.
[viii].D. DAVOR, J. GEARHART, Putting Stem Cells to Work, Science 1999, 283,
[ix].Cf. C. S. POTTEN (ed.), Stem Cells, Academic Press, London 1997, p. 474;
D. ORLIC, T. A. BOCK, L. KANZ, Hemopoietic Stem Cells: Biology and
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F. PITTENGER, A. M. MACKAY, S.C. BECK et al., Multilineage Potential of Adult
Human Mesenchymal Stem Cells, Science 1999, 284, 143-147; C. R. R. BJORNSON,
R.L. RIETZE, B. A. REYNOLDS et al., Turning Brain into Blood: a Hematopoietic
Fate Adopted by Adult Neural Stem Cells in vivo, Science 1999, 283, 534-536; V.
OUREDNIK, J. OUREDNIK, K. I. PARK, E. Y. SNYDER, Neural Stem Cells - a Versatile
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Clinical Genetics 1999, 56, 267-278; I. LEMISCHKA, Searching for Stem Cell
Regulatory Molecules: Some General Thoughts and Possible Approaches, Ann. N.Y.
Acad. Sci. 1999, 872, 274-288; H. H. GAGE, Mammalian Neural Stem Cells, Science
2000, 287, 1433-1438; D. L. CLARKE, C. B. JOHANSSON, J. FRISEN et al.,
Generalized Potential of Adult Neural Stem Cells, Science 2000, 288, 1660-1663;
G. VOGEL, Brain Cells Reveal Surprising Versatility, ibid., 1559-1561.
[x].Cf. R. L. PHILLIPS, R. E. ERNST, I. R. LEMISCHKA, et al., The Genetic
Program of Hematopoietic Stem Cells, Science 2000, 288, 1635-1640.
[xi].Cf. D. J. WATT, G. E. JONES, Skeletal Muscle Stem Cells: Function and
Potential Role in Therapy, in C. S. POTTEN, Stem Cells, op. cit., 75-98; J. A.
NOLTA, D. B. KOHN, Haematopoietic Stem Cells for Gene Therapy, ibid., 447-460;
Y. REISNER, E. BACHAR-LUSTIG, H-W. LI et al., The Role of Megadose CD34+
Progenitor Cells in the Treatment of Leukemia Patients Without a Matched Donor
and in Tolerance Induction for Organ Transplantation, Ann. N.Y. Acad. Sci. 1999,
872, 336-350; D. W. EMERY, G. STAMATOYANNOPOULOS, Stem Cell Gene Therapy for
the ß-Chain Hemoglobinopathies, ibid., 94-108; M. GRIFFITH, R. OSBORNE, R.
MUNGER, Functional Human Corneal Equivalents Constructed from Cell Lines,
Science 1999, 286, 2169-2172; N. S. ROY, S. WANG, L. JIANG et al., In vitro
Neurogenesis by Progenitor Cells Isolated from the Adult Hippocampus, Nature
Medicine 2000, 6, 271-277; M. NOBLE, Can Neural Stem Cells Be Used as
Therapeutic Vehicles in the Treatment of Brain Tumors?, ibid., 369-370; I. L.
WEISSMAN, Translating Stem and Progenitor Cell Biology to the Clinic: Barriers
and Opportunities, Science 2000, 287, 1442-1446; P. SERUP, Panning for
Pancreatic Stem Cells, Nature Genetics 2000, 25, 134-135.
[xii].E. MARSHALL, The Business of Stem Cells, Science 2000, 287, 1419-1421.
[xiii].Cf. O. BRUSTLE, K. N. JONES, R. D. LEARISH et al., Embryonic Stem
Cell-Derived Glial Precursors: a Source of Myelinating Transplants, Science
1999, 285, 754-756; J. W. McDONALD, X-Z LIU, Y. QU et al., Transplanted
Embryonic Stem Cells Survive, Differentiate and Promote Recovery in Injured Rat
Spinal Cord, Nature Medicine 1999, 5, 1410-1412.
[xiv].Cf. A. SERRA , R. COLOMBO, Identità e Statuto dell'Embrione Umano: il
Contributo della Biologia, in PONTIFICIA ACADEMIA PRO VITA, Identità e Statuto
dell'Embrione Umano, Libreria Editrice Vaticana, Città del Vaticano 1998,
[xv].Cf. I. CARRASCO de PAULA, Il Rispetto Dovuto all'Embrione Umano:
Prospettiva Storico-Dottrinale, in ibid., pp. 9-33; R. LUCAS LUCAS, Statuto
Antropologico dell'Embrione Umano, in ibid., pp.159-185; M. COZZOLI, L'Embrione
Umano: Aspetti Etico-Normativi, in ibid., pp.237- 273; L. EUSEBI, La Tutela
dell'Embrione Umano: Profili Giuridici, in ibid., pp. 274-286.
[xvi].JOHN PAUL II, Encyclical Letter "Evangelium Vitae" (25 March 1995),
Acta Apostolicae Sedis 1995, 87, 401-522; cf. also CONGREGATION FOR THE DOCTRINE
OF THE FAITH, Instruction on Respect for Human Life in Its Origins and on the
Dignity of Procreation "Donum Vitae" (22 February 1987), Acta Apostolicae Sedis
1988, 80, 70-102.
[xvii].CONGREGATION FOR THE DOCTRINE OF THE FAITH, op. cit., I, no. 6;
C.B.COHEN (ed.), Special Issue: Ethics and the Cloning of Human Embryos, Kennedy
Institute of Ethics Journal 1994, n.4, 187-282; H. T. SHAPIRO, Ethical and
Policy Issues of Human Cloning, Science 1997, 277, 195-196; M.L. DI PIETRO,
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6, 1099-2005; A. SERRA, Verso la Clonazione dell'Uomo? Una Nuova Frontiera della
Scienza, La Civiltà Cattolica 1998 I, 224-234; ibid., La Clonazione Umana in
Prospettiva "Sapienziale", ibid., 329-339.